fit2x

General description

fits2x is a collection of fit models that use maximum likelihood estimators for polarization and time-resolved fluorescence decays (see [MCH+01]).

fit2x implement burst integrated fluorescence lifetime fits (BIFL) with scatter. The library can be used in conjuncture with tttrlib to analyze and process single-molecule FRET (smFRET) experiments and confocal laser scanning microscopy (CLSM) data. The fit2x shared libary can be used from LabView and is wrapped by SWIG (Simplified Wrapper and Interface Generator) for common scripting languages as Python as main target language.

## Design goals * Low memory footprint (keep objective large datasets, e.g. FLIM in memory). * Platform independent C/C++ library with interfaces for scripting libraries

## Capabilities * Polarization and time-resolved analysis * Stable analysis results with minimum photon counts * Robust thoroughly tested maximum likelihood estimators

Documentation

Documentation for the latest releases is available here,. Previous releases are available through the read-the-docs page page,.

Indices and tables

License

fit2x was developed at the Seidel Lab (Heinrich Heine University) and is maintained by Thomas Peulen. fit2x is released under the open source MIT license.